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Product Name
PHD1 Rabbit mAb
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Gene ID
112398
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SwissProt ID
Q96KS0
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Gene Name
EGLN2
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Alternative Names
Estrogen-induced tag 6; HPH-3; PHD1
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Background
Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
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Research Field
Cardiovascular
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Product Categories
Recombinant Rabbit Monoclonal antibody
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Host
Rabbit
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Reactivity
Human,Rat
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Application
WB
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Dilution Ratio
WB: 1/500-1/1000
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Molecular Weight
Calculated MW: 44 kDa; Observed MW: 44 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
R01-7G1
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Isotype
IgG
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Immunogen
A synthesized peptide derived from human PHD1/prolyl hydroxylase
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.