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Product Name
SC35 Rabbit mAb
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Gene ID
6427
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SwissProt ID
Q01130
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Gene Name
SRSF2
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Alternative Names
Protein PR264; Splicing component; 35 kDa; Splicing factor SC35; SC-35; Splicing factor; arginine/serine-rich 2
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Background
Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment.
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Research Field
Epigenetics and Nuclear Signaling
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Product Categories
Recombinant Rabbit Monoclonal antibody
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Host
Rabbit
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Reactivity
Human,Rat
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Application
WB,IHC-P,IP
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Dilution Ratio
WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
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Molecular Weight
Calculated MW: 25 kDa; Observed MW: 35 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
R04-7J2
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Isotype
IgG
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Immunogen
A synthetic peptide of human SC35
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.