-
Product Name
IDH1(R132S Mutant) Mouse mAb
-
SwissProt ID
-
Gene Name
IDH1, IDCD, IDH, IDP, IDPC, PICD
-
Alternative Names
IDH1, IDCD, IDH, IDP, IDPC, PICD
-
Background
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.
-
Research Field
Signal Transduction
-
Product Categories
Primary antibody
-
Host
Mouse
-
Reactivity
Vertebrates
-
Application
WB, IHC-P, ICC/IF, ELISA
-
Dilution Ratio
ELISA: 1/1000-1/5000 WB: 1/500-1/1000 ICC/IF: 1/50-1/100 IHC-P: 1/50-1/100
-
Clonality
Monoclonal Antibody
-
Clonality No.
2W2-A6-P7
-
Isotype
IgG
-
Immunogen
A synthetic peptide from the internal region of IDH1 which includes the mutation of R132S, human origin.
-
Purification
Purified from ascites
-
Conjugation
Unconjugated
-
Modification
Unmodified
-
Form
Liquid
-
Buffer System
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
-
Storage
Store at -20°C. Avoid repeated freezing and thawing