-
Product Name
Pepsinogen 5 (1G8) Mouse mAb
-
Gene ID
5222
-
SwissProt ID
P0DJD9
-
Gene Name
PGA5
-
Alternative Names
Pepsinogen 5; Pepsin A
-
Background
PGA5: Pepsinogen 5, group I (pepsinogen A). Pepsinogens are the inactive precursors of pepsin, the major acid protease found in the stomach. Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor,pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity (pH 1-3) for pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. PGA5 is a member of the subfamily A1 within the pepsin family and is the predominant endopeptidase in the gastric juice of vertebrates.PGA5 is inhibited by ovUS-1, a uterine serpin.
-
Research Field
Cell Biology
-
Product Categories
Primary antibody
-
Host
Mouse
-
Reactivity
Human
-
Application
WB,IHC-P
-
Dilution Ratio
WB: 1/500-1/1000 IHC: 1/100-1/200
-
Molecular Weight
Calculated MW: 42 kDa; Observed MW: 42 kDa
-
Clonality
Monoclonal Antibody
-
Clonality No.
1G8-D7-F0
-
Isotype
IgG1
-
Immunogen
Purified recombinant fragment of human PGA5 expressed in E. Coli.
-
Purification
Ascitic Fluid
-
Conjugation
Unconjugated
-
Modification
Unmodified
-
Form
Liquid
-
Buffer System
Ascitic fluid containing 0.03% sodium azide.
-
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.