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Product Name
LC3A (8F5) Mouse mAb
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Gene ID
84557
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SwissProt ID
Q9H492
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Gene Name
MAP1LC3A
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Alternative Names
Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha
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Background
Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form.
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Research Field
Signal Transduction
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Product Categories
Primary antibody
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Host
Mouse
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Reactivity
Human,Rat,Mouse
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Application
WB,IHC-P
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Dilution Ratio
WB: 1/500-1/1000 IHC: 1/50-1/100
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Molecular Weight
Calculated MW: 14 kDa; Observed MW: 14,16 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
8F5-10H1-9A7
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Isotype
IgG1
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Immunogen
Purified recombinant protein expressed in E.coli.
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.