产品详情
-
Product Name
CPSF4 Rabbit pAb
-
Gene ID
10898
-
SwissProt ID
O95639
-
Gene Name
CPSF4
-
Alternative Names
NAR; NEB1; CPSF30
-
Background
Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene.
-
Research Field
Microbiology
-
Product Categories
Primary antibody
-
Host
Rabbit
-
Reactivity
Human,Mouse,Rat
-
Application
WB
-
Dilution Ratio
WB: 1/500-1/1000
-
Molecular Weight
Calculated MW: 30 kDa; Observed MW: 30 kDa
-
Clonality
Polyclonal Antibody
-
Clonality No.
-
-
Isotype
IgG
-
Immunogen
Fusion protein of human CPSF4
-
Purification
Affinity Purified
-
Conjugation
Unconjugated
-
Modification
Unmodified
-
Form
Liquid
-
Buffer System
pH 7.4 PBS, 0.05% NaN3, 40% Glycerol
-
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
应用缩写
WB:蛋白质印迹法IHC-P:石蜡切片样本的免疫组织化学法IHC-F:冰冻切片样本的免疫组织化学法ICC/IF:免疫细胞荧光 IP:免疫沉淀法FC:流式细胞术ChIP:染色质免疫沉淀法ELISA:酶联免疫吸附测定
正能生物产品售后保障范围仅限官网产品详情页中“应用”一栏写明的实验应用，请参照上方的缩写含义，仔细对照后选择合适的产品。