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Product Name
Hexokinase I Mouse mAb
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Gene ID
3098
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SwissProt ID
P19367
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Gene Name
HK1
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Alternative Names
HKI; HXK1; HK1-ta; HK1-tb; HK1-tc; HK1
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Background
The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose- 6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines.
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Research Field
Cardiovascular
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Product Categories
Primary antibody
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Host
Mouse
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Reactivity
Human,Rat,Mouse
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Application
WB,IHC-P,ICC/IF
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Dilution Ratio
WB: 1/500-1/1000 IHC: 1/100-1/200 IF: 1/50-1/200
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Molecular Weight
Calculated MW: 102 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
4B2-U5-H7
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Isotype
Mouse IgG1
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Immunogen
Purified recombinant fragment of human HK1 expressed in E. Coli.
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Ascitic fluid containing 0.03% sodium azide.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.