-
Product Name
CD95 Mouse mAb
-
Gene ID
355
-
SwissProt ID
P25445
-
Gene Name
FAS
-
Alternative Names
APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
-
Background
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.
-
Research Field
Cell Biology
-
Product Categories
Primary antibody
-
Host
Mouse
-
Reactivity
Human
-
Application
WB,FC,ELISA
-
Dilution Ratio
WB: 1/500-1/1000 FC: 1/50-1/100 ELISA: 1/10000
-
Molecular Weight
Calculated MW: 38 kDa;Observed MW: 55 kDa
-
Clonality
Monoclonal Antibody
-
Clonality No.
4P8-E5-V3
-
Isotype
Mouse IgG1
-
Immunogen
Purified recombinant fragment of human FAS expressed in E. Coli.
-
Purification
Affinity Purified
-
Conjugation
Unconjugated
-
Modification
Unmodified
-
Form
Liquid
-
Buffer System
Purified antibody in PBS with 0.05% sodium azide
-
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.