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Product Name
Neutrophil Elastase Mouse mAb
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Gene ID
1991
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SwissProt ID
P08246
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Gene Name
ELANE
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Alternative Names
GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
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Background
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.
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Research Field
Immunology
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Product Categories
Primary antibody
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Host
Mouse
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Reactivity
Human,Monkey
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Application
WB,IHC-P,FC,ELISA
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Dilution Ratio
WB: 1/500-1/1000 IHC: 1/100-1/200 FC: 1/50-1/100 ELISA: 1/10000
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Molecular Weight
Calculated MW: 29 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
3V5-T6-T3
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Isotype
Mouse IgG1
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Immunogen
Purified recombinant fragment of human ELANE expressed in E. Coli.
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Purified antibody in PBS with 0.05% sodium azide
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.