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Product Name
DNA PKcs (8D3) Mouse mAb
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Gene ID
5591
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SwissProt ID
P78527
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Gene Name
PRKDC
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Alternative Names
PRKDC; HYRC; HYRC1; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; p460
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Background
The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.
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Research Field
Epigenetics and Nuclear Signaling
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Product Categories
Primary antibody
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Host
Mouse
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Reactivity
Human
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Application
WB,ICC/IF,IP
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Dilution Ratio
WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20
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Molecular Weight
Calculated MW: 469 kDa; Observed MW: 450 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
8D3-B12-F11
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Isotype
IgG2b
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Immunogen
Purified recombinant human DNA-PKcs protein fragments expressed in E.coli
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.