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Product Name
GCSm-γ Rabbit pAb
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Gene ID
2730
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SwissProt ID
P48507
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Gene Name
GCLM
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Alternative Names
Glutamate--cysteine ligase regulatory subunit; GCS light chain; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; Glutamate--cysteine ligase modifier subunit; GLCLR; GCLM
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Background
Gamma-glutamylcysteine synthetase (γ-GCS) is the rate limiting enzyme for glutathione (L-γ-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. γ-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic ntioxidants, heat shock, oxidants and cytokines. The human γ-GCS gene encoding the 367 amino acid catalytic subunit maps to chromosome 6p12. The human γ-GCS gene encoding the regulatory subunit maps to chromosome 1p22-p21. The two subunits of γ-GCS form a heterodimeric zinc metalloprotein that gains activity through formation of a reversible disulfide bond.
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Research Field
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Product Categories
Primary antibody
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Host
Rabbit
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Reactivity
Human,Mouse,Rat
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Application
WB, IHC-P
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Dilution Ratio
WB: 1/500-1/1000 IHC: 1/50-1/200
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Molecular Weight
Calculated MW: 30kDa
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Clonality
Polyclonal Antibody
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Clonality No.
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Isotype
IgG
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Immunogen
Synthetic peptide corresponding to Human GCSm-γ.
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.