产品详情
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Product Name
LexA DNA Binding Region Rabbit pAb
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Gene ID
948544
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SwissProt ID
P0A7C2
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Gene Name
lexA
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Alternative Names
lexa dna binding region
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Background
Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment.
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Research Field
Microbiology
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Product Categories
Primary antibody
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Host
Rabbit
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Reactivity
Cyanobacteria
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Application
WB
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Dilution Ratio
WB: 1/500-1/1000
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Molecular Weight
Refer to figures
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Clonality
Polyclonal Antibody
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Clonality No.
-
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Isotype
IgG
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Immunogen
Recombinant Protein of LexA DNA Binding Region
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
应用缩写
WB:蛋白质印迹法IHC-P:石蜡切片样本的免疫组织化学法IHC-F:冰冻切片样本的免疫组织化学法ICC/IF:免疫细胞荧光 IP:免疫沉淀法FC:流式细胞术ChIP:染色质免疫沉淀法ELISA:酶联免疫吸附测定
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