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Product Name
Phospho-DARPP32 (Thr75) Rabbit pAb
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Gene ID
84152
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SwissProt ID
Q9UD71
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Gene Name
PPP1R1B
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Alternative Names
Protein phosphatase 1 regulatory subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein; PPP1R1B; DARPP32
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Background
Dopaminergic signaling pathways, which are essential for multiple brain functions, are abnormal in several neurological disorders, such as schizophrenia, Parkinson’s disease and drug abuse. DARPP-32 (for dopamine and adenosine 3’,5’-monophosphate-regulated phosphoprotein of 32 kDa) is abundant in neurons that receive dopaminergic input. Activation of PKA and the consequent phosphorylation of DARPP-32 on threonine occurs in response to dopamine acting upon D1-like receptors. Dopamine interaction with D2-like receptors results in the inhibition of PKA activation, the activation of protein phosphatase 2B and the consequent dephosphorylation of DARPP-32. Neurotransmitters other than dopamine may also be able to stimulate the phosphorylation or dephosphorylation of DARPP-32. Phosphorylated DARPP-32 is a potent inhibitor of PP-1.
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Research Field
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Product Categories
Primary antibody
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Host
Rabbit
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Reactivity
Human,Mouse,Rat
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Application
WB, IHC-P
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Dilution Ratio
WB: 1/500-1/1000 IHC: 1/50-1/200
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Molecular Weight
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Clonality
Polyclonal Antibody
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Clonality No.
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Isotype
IgG
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Immunogen
Synthetic phosphopeptide derived from human DARPP-32 around the phosphorylation site of Threonine 75.
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Phosphorylated
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Form
Liquid
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Buffer System
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.