-
Product Name
Phospho-PRAS40 (Thr246) Rabbit pAb
-
Gene ID
84335
-
SwissProt ID
Q96B36
-
Gene Name
AKT1S1
-
Alternative Names
Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate; AKT1S1; PRAS40
-
Background
Akt, also known as protein kinase B, is one of the major downstream targets of the phosphatidylinositol 3-kinase pathway. This protein kinase has been implicated in insulin signaling, stimulation of cellular growth, inhibition of apoptosis and transformation of cells. The proline-rich Akt substrate PRAS40, also designated AKT1S1, becomes phosphorylated by activated Akt on Ser or Thr residues in the motif RXRXX(S/T). Phosphorylated PRAS40 subsequently binds 14-3-3 in a sequence-specific manner, thereby inducing such changes as alteration of protein subcellular localization and regulation of intrinsic enzymatic activity. Studies also suggest that PRAS40 phosphorylation and its interaction with pAkt and 14-3-3 may play an important role in neuroprotection mediated by NGF in apoptotic neuronal cell death after cerebral ischemia. PRAS40 maps to human chromosome 19q13.33.
-
Research Field
-
-
Product Categories
Primary antibody
-
Host
Rabbit
-
Reactivity
Human,Mouse,Rat
-
Application
WB
-
Dilution Ratio
WB: 1/500-1/1000
-
Molecular Weight
-
-
Clonality
Polyclonal Antibody
-
Clonality No.
-
-
Isotype
IgG
-
Immunogen
Synthetic phosphopeptide derived from human PRAS40 around the phosphorylation site of Threonine 246.
-
Purification
Affinity Purified
-
Conjugation
Unconjugated
-
Modification
Phosphorylated
-
Form
Liquid
-
Buffer System
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
-
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.