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Product Name
EIF3E Rabbit pAb
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Gene ID
3646
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SwissProt ID
P60228
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Gene Name
EIF3E
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Alternative Names
Eukaryotic translation initiation factor 3 subunit E, eIF3e, Eukaryotic translation initiation factor 3 subunit 6 , Viral integration site protein INT-6 homolog, eIF-3 p48, EIF3E
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Background
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl- tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation . The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression . Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway . May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.
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Research Field
Epigenetics and Nuclear Signaling
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Product Categories
Primary antibody
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Host
Rabbit
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Reactivity
Human,Zebrafish
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Application
WB
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Dilution Ratio
WB: 1/1000
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Molecular Weight
Calculated MW:52 kDa; Observed MW: 40-55 kDa
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Clonality
Polyclonal Antibody
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Clonality No.
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Isotype
IgG
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Immunogen
This EIF3E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide from the Central region of human EIF3E.
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Liquid in PBS containing 0.09% sodium azide, pH 7.3.
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Storage
Store at 2-8°C for up to 2 weeks. store at -20°C for long term storage in small aliquots. Avoid freeze/thaw cycles.