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Product Name
Phospho-IκB-α (Tyr305) Rabbit pAb
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Gene ID
4792
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SwissProt ID
P25963
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Gene Name
NFKBIA
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Alternative Names
I-kappa-B-alpha; IkappaBalpha; IKBA; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappaB inhibitor alpha; NFKBI; NFKBIA; RL/IF-1
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Background
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed:1493333, PubMed:36651806, PubMed:7479976).
On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed:7479976, PubMed:7628694, PubMed:7796813, PubMed:7878466).
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Research Field
Signal Transduction
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Product Categories
Primary antibody
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Host
Rabbit
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Reactivity
Human,Mouse,Rat
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Application
WB,IHC-P
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Dilution Ratio
WB: 1/500-1/3000 IHC: 1/50-1/100
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Molecular Weight
Calculated MW: 35 kDa
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Clonality
Polyclonal Antibody
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Isotype
IgG
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Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human IκB-α around the phosphorylation site of tyrosine 305 (L-P-YP-D-D).
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Phosphorylated
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Form
liquid
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Buffer System
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.