-
Product Name
Phospho-BLK (Tyr501) Rabbit pAb
-
Gene ID
640
-
SwissProt ID
P51451
-
Gene Name
BLK
-
Alternative Names
B lymphocyte kinase; EC 2.7.10.2; kinase Blk; p55-BLK; Tyrosine-protein kinase BLK
-
Background
Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling (By similarity).
B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors (By similarity).
Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation (By similarity).
Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor (By similarity).
Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr-207' (By similarity).
Phosphorylates also the immunoglobulin G receptors FCGR2A, FCGR2B and FCGR2C (PubMed:8756631).
-
Research Field
Cell Biology
-
Product Categories
Primary antibody
-
Host
Rabbit
-
Reactivity
Human,Mouse,Rat
-
Application
IHC-P
-
Dilution Ratio
IHC: 1/50-1/100
-
Molecular Weight
Calculated MW: 57 kDa
-
Clonality
Polyclonal Antibody
-
Isotype
IgG
-
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human BLK around the phosphorylation site of tyrosine 501 (R-Q-YP-E-L).
-
Purification
Affinity Purified
-
Conjugation
Unconjugated
-
Modification
Phosphorylated
-
Form
liquid
-
Buffer System
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
-
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.