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Product Name
Phospho-Bloom Syndrome (Thr99) Rabbit pAb
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Gene ID
641
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SwissProt ID
P54132
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Gene Name
BLM
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Alternative Names
Bloom's syndrome protein, DNA helicase, RecQ-like, type 2, EC 3.6.1.-, RECQ2, RECQL3, RecQ protein-like 3
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Background
ATP-dependent DNA helicase that unwinds double-stranded (ds)DNA in a 3'-5' direction (PubMed:24816114, PubMed:25901030, PubMed:9388193, PubMed:9765292).
Participates in DNA replication and repair (PubMed:12019152, PubMed:21325134, PubMed:23509288, PubMed:34606619).
Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA (PubMed:21325134).
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Research Field
Epigenetics and Nuclear Signaling
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Product Categories
Primary antibody
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Host
Rabbit
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Reactivity
Human
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Application
WB,IHC-P,ICC/IF
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Dilution Ratio
WB: 1/500-1/3000 IHC: 1/50-1/100 ICC/IF: 1/100-1/500
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Molecular Weight
Calculated MW: 159 kDa
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Clonality
Polyclonal Antibody
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Isotype
IgG
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Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Bloom Syndrome Protein around the phosphorylation site of threonine 99 (Q-E-TP-Q-R).
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Phosphorylated
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Form
liquid
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Buffer System
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.