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Product Name
Phospho-NFAT5/TonEBP (Ser155) Rabbit pAb
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Gene ID
10725
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SwissProt ID
O94916
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Gene Name
NFAT5
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Alternative Names
KIAA0827; NF-AT5; nuclear factor of activated T cells 5; T cell transcription factor NFAT5; TonE-binding protein; TonEBP; tonicity-responsive enhancer-binding protein
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Background
Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3' (PubMed:10377394).
Mediates the transcriptional response to hypertonicity (PubMed:10051678).
Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17 (PubMed:22266867).
Also involved in the DNA damage response by preventing formation of R-loops; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA (PubMed:34049076).
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Research Field
Immunology
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Product Categories
Primary antibody
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Host
Rabbit
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Reactivity
Human,Mouse
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Application
WB
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Dilution Ratio
WB: 1/500-1/3000
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Molecular Weight
Calculated MW: 165 kDa
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Clonality
Polyclonal Antibody
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Isotype
IgG
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Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human NFAT5/TonEBP around the phosphorylation site of serine 155 (D-N-SP-R-M).
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Phosphorylated
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Form
liquid
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Buffer System
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.