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Product Name
Phospho-Centromeric Protein A (Ser7) Rabbit pAb
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Gene ID
1058
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SwissProt ID
P49450
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Gene Name
CENPA
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Alternative Names
CENA, CENP-A, CENPA, Centromere autoantigen A, Centromere protein A
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Background
Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes (PubMed:11756469, PubMed:14667408, PubMed:15282608, PubMed:15475964, PubMed:15702419, PubMed:17651496, PubMed:19114591, PubMed:20739937, PubMed:27499292, PubMed:7962047, PubMed:9024683).
Replaces conventional H3 in the nucleosome core of centromeric chromatin that serves as an assembly site for the inner kinetochore (PubMed:18072184).
The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3 (PubMed:26878239, PubMed:27499292).
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Research Field
Epigenetics and Nuclear Signaling
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Product Categories
Primary antibody
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Host
Rabbit
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Reactivity
Human
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Application
ICC/IF
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Dilution Ratio
ICC/IF: 1/100-1/500
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Molecular Weight
Calculated MW: 15 kDa
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Clonality
Polyclonal Antibody
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Isotype
IgG
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Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Centromeric Protein A around the phosphorylation site of serine 7 (R-R-SP-R-K).
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Phosphorylated
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Form
liquid
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Buffer System
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.