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Product Name
Phospho-c-Met (Tyr1003) Rabbit pAb
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Gene ID
4233
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SwissProt ID
P08581
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Gene Name
MET
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Alternative Names
EC 2.7.10.1, HGF receptor, HGF-SF receptor, Hepatocyte growth factor receptor precursor, Met proto- oncogene tyrosine kinase, c-met, kinase EC 2.7.10.1, HGF receptor, HGF-SF receptor, Hepatocyte growth factor receptor precursor, Met proto- oncogene tyrosi
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Background
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of neuronal precursors, angiogenesis and kidney formation. During skeletal muscle development, it is crucial for the migration of muscle progenitor cells and for the proliferation of secondary myoblasts (By similarity).
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Research Field
Signal Transduction
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Product Categories
Primary antibody
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Host
Rabbit
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Reactivity
Human,Mouse,Rat
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Application
WB,IHC-P,ICC/IF
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Dilution Ratio
WB: 1/500-1/3000 IHC: 1/50-1/100 ICC/IF: 1/100-1/500
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Molecular Weight
Calculated MW: 155 kDa
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Clonality
Polyclonal Antibody
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Isotype
IgG
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Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human c-Met around the phosphorylation site of tyrosine 1003 (V-D-YP-R-A).
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Phosphorylated
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Form
liquid
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Buffer System
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.