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Product Name
Phospho-Retinoblastoma (Ser608) Rabbit pAb
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Gene ID
5925
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SwissProt ID
P06400
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Gene Name
RB1
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Alternative Names
P105-RB; PP105; PP110; RB-1; RB1; retinoblastoma gene; Retinoblastoma-associated protein
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Background
Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802).
The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802).
Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802).
Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed:10499802).
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Research Field
Cell Biology
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Product Categories
Primary antibody
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Host
Rabbit
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Reactivity
Human,Mouse,Rat
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Application
WB,IHC-P
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Dilution Ratio
WB: 1/500-1/3000 IHC: 1/50-1/100
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Molecular Weight
Calculated MW: 106 kDa
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Clonality
Polyclonal Antibody
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Isotype
IgG
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Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Retinoblastoma around the phosphorylation site of serine 608 (Y-L-SP-P-V).
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Phosphorylated
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Form
liquid
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Buffer System
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.