-
Product Name
Phospho-RFWD2 (Ser387) Rabbit pAb
-
Gene ID
64326
-
SwissProt ID
Q8NHY2
-
Gene Name
RFWD2
-
Alternative Names
Constitutive photomorphogenesis protein 1 homolog; COP1; EC 6.3.2.-; hCOP1; RING finger and WD repeat domain protein 2; Ubiquitin- protein ligase COP1
-
Background
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable).
-
Research Field
Epigenetics and Nuclear Signaling
-
Product Categories
Primary antibody
-
Host
Rabbit
-
Reactivity
Human,Mouse
-
Application
WB
-
Dilution Ratio
WB: 1/500-1/3000
-
Molecular Weight
Calculated MW: 80 kDa
-
Clonality
Polyclonal Antibody
-
Isotype
IgG
-
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human RFWD2 around the phosphorylation site of serine 385 (T-A-SP-Q-L).
-
Purification
Affinity Purified
-
Conjugation
Unconjugated
-
Modification
Phosphorylated
-
Form
liquid
-
Buffer System
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
-
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.